Dr. Daleke's interests lie in the generation and maintenance of transmembrane phospholipid asymmetry which are essential for the function of biological membranes, yet the mechanism underlying these fundamental processes remains unclear. Our research effort is designed to understand how phospholipids are assembled in biological membranes and how the resulting phospholipid asymmetry is maintained. Phospholipid transporters, or "flippases," such as the recently discovered aminophospholipid translocator, represent an interesting new class of proteins that may play a key role in the assembly and organization of phospholipids in biological membranes. These enzymes require energy in the form of ATP, have strict phospholipid structural requirements, and are unique in their ability to transport lipids across membranes. Our goal is to elucidate the structure, function, and biological significance of these proteins.
Affiliations
- American Society for Biochemistry and Molecular Biology
- American Association for the Advancement of Science
- American Chemical Society
- Biophysical Society
- American Heart Association
Awards and Honors
- Indiana Univeristy School of Medicine Eminent Scholar, 1999
- Established Investigator, American Heart Association, 1994-1999
- Trustees Teaching Excellence Award, 1999
Publications
Articles (since 2000)
- David L. Daleke. (2008). Regulation of Phospholipid Assymetry in the Erythrocyte Membrane. Current Opinion in Hematology 15:191-195.
- David L. Daleke. (2007). Phospholipid Flippases. Journal of Biological Chemistry 282:821-825.
- Smriti and David L. Daleke. (2007). ATP-dependent Transport of Phosphatidylserine Analogs in Human Erythrocytes. Biochemistry 46:2249-2259.
- Jill Paterson, Kathleen Renkema, Maragaret Halleck, Robert Schlegel, Patrick Williamson and David L. Daleke. (2006). Lipid Specific Activation of the Murine P4-ATPase Atp8a1 (ATPase II). Biochemistry 45:5367-5376.
- Dana M. Niedowicz and David L. Daleke. (2005). The Role of Oxidative Stress in Diabetic Complications. Cell Biochemistry and Biophysics 43:289-330.
- Peter R. Hoffmann, Jennifer A. Kench, Andrea Vondracek, Ellen Kruk, David L. Daleke, Michael Jordan, Philippa Marrack, Peter M. Henson, and Valerie A. Fadok. (2005). Interaction between Phosphatidylserine and the Phosphatidylserine Receptor Inhibits Immune Responses In Vivo. J Immunol 174:1393-1404.
- Jill V. Lyles, Kathleen Cornely-Moss, Christine M. Smith and David L. Daleke. (2003). Purification of the Human Erythrocyte PS-Stimulated Mg2+-ATPase: A Putative Flippase. Methods in Molecular Biology 228:257-269.
- David L. Daleke. (2003). Regulation of Transbilayer Plasma Membrane Phospholipid Asymmetry. Journal of Lipid Reearch 44:233-242.
- Mou Banerjee, Daryl C. Drummond, Arvind Srivastava, David L. Daleke, and Barry R. Lentz. (2002). Specificity of Soluble Phospholipid Binding Sites on Human Factor Xa. Biochemistry 41:7751-7762.
- Xin Zhai, Arvind Srivastava, Daryl C. Drummond, David Daleke, and Barry R. Lentz. (2002). Phosphatidylserine Binding Alters the Conformation and Specifically Enhances the Cofactor Activity of Bovine Factor Va. Biochemistry 41:5675-5684.
- Peter R. Hoffman, Aimee M. deCathelineau, Carol Anne Ogden, Yann Leverrier, Donna L. Bratton, David L. Daleke, Anne J. Ridley, Valerie A. Fadok, and Peter M. Henson. (2001). Phosphatidylserine (PS) Induces PS receptor-mediated Macropinocytosis and Promotes Clearance of Apoptotic Cells. Journal of Cell Biology 155:649-659.
- Valerie A. Fadok, Aime de Cathelineau, David L. Daleke, Peter M. Henson and Donna L. Bratton. (2001). Loss of Phospholipid Asymmetry and Surface Exposure of Phosphatidylserine is Required for Phagocytosis of Apoptotic Cells by Macrophages and Fibroblasts. Journal of Biological Chemistry 276:1071-1077.
- Umakant J. Dumaswala, Michael J. Wilson, Y. L. Wu, J. Wykle, L. Zhou, L. M. Douglass, and David L. Daleke. (2000). Glutathione Loading Prevents Free Radical Injury in Red Blood Ceells After Storage. Free Radical Research 33:517-529.
- David L. Daleke and Jill V. Lyles. (2000). Identification and Purification of Phospholipid Flippases. Biochimica et Biophysica Acta 1486:108-127.
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